socalfunplaces Chapter 459
Chapter 459
In any case, the low-temperature baking conditions have been achieved, and Lu Zhe will prepare the reagent for extracting colchicine.
It can be used as a substance that alkalizes colchicine, as well as an extractor!
Now, the most readily available alkalizing substance is ammonia.
Similarly, suitable as an extractant is ethanol, commonly known as alcohol.
The two solvents are almost identical, both are distilled, the only difference is the raw material.
This is done by distilling the raw materials in sealed glass bottles, leaving only a thin tube on top to pass through another bottle to prevent alcohol or ammonia from volatilizing.
If the raw material used is wine, alcohol will be miscible with condensed water vapor to form an ethanol solution.
At the same time, if shark skin or urine is used, ammonia will also be miscible with water again because it cannot be transpired, forming ammonia.
Lu Zhe said the specific method to the shallow, shallow is not so sad, said that he has learned new knowledge and is looking forward to it.
Lu Zhe feels that shallow is really a girl with a good personality, has a good temper, and is easy to coax, and marries home and will not quarrel with you for the rest of her life.
But when he saw Lu Zhe take the shark skin, he was a little more disappointed.
Then, Lu Zhe began to operate, because he had some experience in distilling baijiu, and this time it was still very successful and made baijiu.
But when steaming shark skin, Lu Zhe found some problems.
Usually sharks excrete bodily fluids from their skin, which contains large amounts of ammonia.
However, as a bull shark with a wide salinity shark, if it lives in the sea for a long time, it is fine, once it lives in fresh water for a long time, the ammonia content in the body will be greatly reduced in order to balance the osmotic pressure.
This is one of the reasons why bull sharks taste better of all sharks.
Therefore, shark skin refining ammonia eventually failed.
There was no way, so Lu Zhe had to retreat to the second place and use another raw material.
Just thinking about who to get some from, he handed out a flared bottle with a shallow smile.
It's still hot, on a cold rainy day, like a little warm baby.
"Who??"
“...... It's mine. Shallow pursed his mouth, and his little face turned into a big apple.
"When was it prepared?"
"Just now! I'm afraid of getting cold and holding it in my arms.
Lu Zhe picked it up, shaking the crystal clear inside with a yellowish liquid, and couldn't help but sigh: "It's really a caring girl!" "
I... I just want to help you!
"Well, we can't live up to your sincerity, let's try this with this!"
Used again, perhaps with the rare characteristics of the goddess itself, as if favored by the goddess of luck, the result was unusually successful, Lu Zhe successfully obtained an ammonia solution.
Before I knew it, 10 hours had passed, and the mud embryo was disassembled, the steamer was opened, and the wild lily slices inside had been dried.
Lu Zhe used a stone scoop to mash these dry flakes into powder, and then put them in ammonia to stir.
"Lu Zhe, what does this mean?"
"This is to use ammonia as a alkalizing reagent, so that after the dried lily powder is alkalized, colchicine will be formed."
"Is this science content?"
"Yes!"
At this time, Ning Shu poked his head out from the cabin: "Hey, what time is it, you two haven't come back to sleep?"
Lu Zhe said: "Today we have important things to do, you should rest first."
"What's important?"
"I'll say it now, you have to ask a lot of questions again, look back at the shallow notes."
"Okay, you two toss, remember to take protective measures."
Lu Zhe was speechless: "Look at us like we are doing something informal?"
Ning Shu looked at it, as if he had indeed misunderstood: "Ahem, I said to do a good job of protection, wear gloves, don't be burned by the fire, haha!" I'm going to sleep! With
that, he went back to the cabin and went to sleep.
Shallow flattened his mouth: "The squad leader bullies!"
Lu Zhe smiled: "She's like that, how to talk about the old saying?" It's called straight, and it's hot. "
That word doesn't fit, does it?"
"I'll tell you when it's old."
Shallow nodded: "Actually... I also like to be bullied by you.
"It's not good to be shallow. It's too late for everyone to love you, if others don't say, you just talk about me, to be fair, when did I bully you? Shallow
looked at the hard-planted wild lilies that were now crushed into powder and soaked in ammonia, and looked at Lu Zhe, who had a sincere face, and shook his head aggrievedly.
"No... You never bully me..."
"That's right, come on, let's continue to extract colchicine."
"That... What's next?
"When lily powder is alkalized, free colchicine is formed, and concentrated colchicine crude products can be obtained by evaporating the solvent. Then you can extract it with ethanol! "
This step is simple, after steaming the ammonia, it forms a pile of coffee like.
"This thing is colchicine?"
"Although it is hard to believe, it does contain colchicine, but such a thing cannot be preserved, we can make a simple Soxhlet extractor."
"What's that?"
"A simple prop for extracting compounds from solid substances!"
"Is it hard to do?"
"It is not difficult, we can replace alcohol lamps with candles, measuring cups with steel helmets, resin as sealants, medical glass bottles, oil pipelines, and plastic bottles instead of extraction bottles, extraction tubes and condensers, and then use siphon to repeatedly purify them."
Shallow listened as if he understood something, and took a small book to memorize.
With the current conditions, these tools are not difficult to obtain, and it only took an hour for Lu Zhe to get it.
"Finally, add ethanol! Because ethanol has strong permeability and will combine with colchicine with hydrogen bonds, it is the best extractor. We should have added 20ml of 70% ethanol, refluxed twice in the water bath, and maintained a temperature of 40 degrees Celsius, but we have limited conditions and can only use distilled spirits with the existing concentration..."
"Well, just look at it."
"Well, it's okay, you can draw a tiger according to the cat when you turn back."
"No, feel free to ask me."
The two people were busy until dawn, the first bottle of colchicine solution, Lu Zhe twisted a little with his fingers, and the information of 0.94% colchicine came out of his mind.
Much lower than laboratory purification, I don't know if it is useful.
But at the same time, a red light comes to mind: "Toxic, please wash your hands quickly!"
Lu Zhe was horrified, quickly ran to the ditch to wash his hands, and asked Shallow to tear another notebook and draw a "skeleton" pattern to paste on it as a warning.
Then, he holds down one end of the straw as a dropper and drops the colchicissus solution on the top of the watermelon, strawberry and rice buds to complete the first infusion of colchicine.
"God willing, I hope to bear more delicious fruit."
He took a shallow look at his only remaining wild lily, and then saw that the large and small pots were full of various plants.
At that moment, she seemed to suddenly understand the meaning of lily's existence!